Preparation of protein extracts from yeast

Hummel and Kula, 1989

1. Collect yeast cells from a 2 x 1 ml culture by centrifugation in 1.5 ml microcentrifuge tubes (1 min at 6000 rpm in microcentrifuge)
2. Remove supernatant
   Resuspend cell pellet in 600 ml TNE
   Add1.2 g of glass beads
3. Mix cell suspension with the MM2000 mixer mill (Retsch, Haan, Germany) at highest oscillation frequency for 6 minutes.
4. Centrifuge the lysate for 10 min at maximal speed in microcentrifuge
5. Recover the above yeast extract for further analysis.

TNE buffer

Glass beads (425 to 600 mm diameter, Sigma)