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Manipulations of E. coli |
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E. coli strains |
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Growth media |
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Transformation of E. coli |
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Transformation by the calcium chloride procedure (modified from Mandel and Higa, ) |
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Transformation by electroporation (Dower et al. ) |
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Transformation of E. coli (Ausubel et al) - quick protocol |
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Preparation of electrocompetente cells (volgens Tung & Chow) |
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Transformation- and mutagenesis-protocol met Epicurian Coli XL-Red |
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Transformation of E. coli (according to Inouoe et al ) |
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Phase contrast microscopy |
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Determination of minimal inhibitory concentration (MIC) of chloramphenicol |
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In vivo karakterisatie van CatI-mutanten |
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Yeast manipulations |
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Saccharomyces cerevisiae strains |
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Growth media for Saccharomyces cerevisiae |
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Determination of cell density |
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Transformation of S. cerevisiae |
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Transformation by the lithium acetate (LiOAc) method (Ito et al, ; Gietz and Shiestl, ) |
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Electroporation of Yeast |
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High efficiency method for LiAc/SS-DNA/PEG transformation of yeast |
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Quick and Easy TRAFO Protocol for yeast transformation |
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Direct transfer of plasmid DNA from yeast to E. coli by electroporation |
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Preparation of protein extracts from yeast |
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Sporulation of yeast |
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Protocol 1: Sporulation in liquid medium (Kassir & Simchen, ) |
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Protocol 2: Sporulation on solid medium (Kassir & Simchen, ) |
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Protocol 3: Sporulation in liquid medium (Codón et al, ) |
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Preparation of mass spores of yeast |
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Tetrad disection of yeast |
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DNA manipulations |
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DNA preparations |
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Mini Plasmid preparation from E. coli cultures (Serghini et al, ) |
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Qiagen Midi (tip ) plasmid preparation from E. coli cultures (Qiagen GmbH, Düsseldorf, Germany) |
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QIAprep Miniprep plasmid purification from E. coli cultures |
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Fast small scale isolation of yeast total DNA (Method of Wach et al.) |
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Fast small scale isolation of total yeast DNA (Hoffman and Winston, ) |
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DNA concentration determination |
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Enzymatic DNA manipulations |
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Purification after ligation |
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Oligonucleotide synthesis |
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Polymerase chain reaction (PCR) |
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Normal PCR |
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Inverse PCR |
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Randommutagenesis using PCR |
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PCR clone analysis on intact E. coli cells |
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DNA sequence analysis |
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Preparation of Sephadex-G |
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Protein manipulations |
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Protein purification |
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Dialysis of CAT-proteins |
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Pefablock |
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Protein concentration determination |
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Total protein concentration |
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Determination of CAT concentration |
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SDS polyacrylamide gel electrophoresis (SDS-PAGE) analysis |
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Determination of protein solubility |
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CAT activity assay |
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Determination of thermal inactivation kinetics |
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Fourier transform infrared (FT-IR) analysis |
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Protocol CAT-ELISA (Boehringer Manheim Biochemica) |
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Overzicht van protocols en ideeën om de denaturatie/renaturatie van eiwitten efficiënter te laten verlopen |
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Isolatieprocedure inclusiepartikels hervouwing van eiwitten uit IB's |
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Software |
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Hoe PDB-coördinaten omzetten in data bruikbaar voor Nanovision ? |