E. coli strains

• WK6: D(lac-proAB) galE straA nal^r [F’ lacI^q lacZDM15 proAB⁺].(Zell and Fritz, 1987)
• GM119: dam3, dcm6, galK2, galT22, lacY1, metB1, mtl1, supE44, thi1, tonA32, tsx78.
  To prepare plasmid DNA that may not be methylated by the dam gene product
• XL1-Red: endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac mutD5 mutS mutT Tn10(Tet^r). (Stratagene, La Jolla, CA)
  To introduce mutations randomly into the target DNA
• XL1-Blue MRF’: D(mrcA)183 D(mcrCB-hsdSMR-mmr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F’ proAB lacI^qZDM15 Tn10(Tet^r)]

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